The research are going to design and production of a number of drugs. There is an increase or discovery of different diseases which causing great harm to society. The past researches indicated that huge number of antibiotics were produced by Gram +ve ike bacteria known as Actinomycetes. So we can say that among all microbes more than 50% of the known antimicrobial compounds were produced by Actinomycetes only. These are a specific type of class of prokaryotes forming thread like structure at some stage of their growth, so refereed as filamentous prokaryotes. This class or group is a actively produce of different types of enzymes, enzyme inhibitors, growth promoter and antibiotics etc. In modern era a number of microbes is getting or developing resistance against different infectious microorganisms (e.g., species of Staphylococcus, Mycobacterium,
) to existing compounds. In our study screening of Actinomycetes was performed by using different river sediments. Soil samples was collected from river Godaveri and Krishna and stored in the U.V. and alcohol sterilized Poly bags. Soil samples was serially diluted upto 10-6
and 1 ml from each dilution was plated on different isolation media like starch Casein agar , Albumin media and YMA media, consisting of antifungal agent Nystatin 50 µg/ ml, by pour plate technique. The plates were incubated at different temperature ranges 18 0
C to 28 0
C upto 7-14 days. There were 3 actinomycetes were isolated and these were streak on solidified Bennet agar media at straight line and plates were kept for incubation in incubator at 370
C for nearly about 3 days. Identification of actinomycetes was performed using Gram’s staining.