2. Multiple Shoot Formation and Efficient Root Induction in Cissus quadrangularis. Poonam Garg, C.P.Malik
A standard protocol for the fast in vitro multiplication and plantlet regeneration of a medicinal plant, Cissus quadrangularis from different in vitro grown explants ( shoot tip, nodal explant and in vivo shoot tip ) is described. All the three explants were cultured on MS medium supplemented with Zeatin alone or in combination with BAP and BAP alone or in combination with adenine sulphate for multiple shoot formation. Of the three explants tested, in vitro nodal explant produced the maximum number of shoots (5 shoots) and the maximum mean shoot length (2.86 cm) were observed on MS medium supplemented with 0.5 mg L-1 Zeatin after 30 days of inoculation. Multiple shoots were obtained from the in vitro shoot tip explant cultured on MS medium supplemented with Zeatin alone at low concentration and BAP in combination with adenine sulphate. Shoot tip cultures of field grown C. quadrangularis proliferated speedily in vitro on MS medium + BAP. For efficient root induction, the in vitro formed shoots were rooted on MS medium supplemented with or without IAA and IBA. The rooted in vitro raised plantlets were acclimatized in pots containing sterile soil having vermicompost at equal quantity and successfully transferred to green house with high survival rate.