Bi-Monthly Publishing Peer Reviewed Journal


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Volume11,Issue5

1. Effect of Growth Regulators on Callus Induction and Organ Formation in Cissus quadrangularis Linn. A Valuable Medicinal Plant
Anand  Manju, Kaur Tejinder
Abstract
Its large scale propagation through conventional methods is beset with many problems and its over exploitation from wild necessitates a need to develop an efficient  micropropagation  protocol  for its large scale multiplication. This valuable plant has not been studied much through in vitro technology and there are not many reports available on callus induction and hardly any report on its organogenic potential.  In the present study, callusing  was effected  from the stem and leaf segments taken from field grown mature plant on MS medium supplemented with different concentrations of Naphthelene Acetic Acid (NAA ) and Kinetin ( Kn) with the highest callus growth occurring on 5.37 µM  NAA and  2.32 µM  Kn. Further addition of 15% CM and 3% glucose (instead of 2% sucrose) to the medium considerably enhanced the callus growth and delayed its browning on subsequent subculturing. The calli formed were yellowish white, highly friable and heterogenous in nature. The histogenetic differentiation in the form of tracheids occurred in all the calli followed by root differentiation on higher concentrations of  NAA( 10.74-21.48 µM )and Kn (4.65 µM) after 4-5 weeks. A low frequency shoot differentiation from leaf callus was observed on NAA (14.7µM) and Kn (2.32µM) after 8 weeks. Direct rooting from leaf and stem segments was observed on MS medium supplemented with different concentrations of auxins like Indole 3 Butyric Acid (IBA) or  NAA either alone or in combination with kinetin.